Sodium aescinate reduces glial fibriallary acidic protein expression after spinal cord injury

doi:10.3969/j.issn.2095-4344.2014.15.020

Abstract

Abstract:

BACKGROUND: The methylprednisolone pulse therapy in early period of spinal cord injury can attenuate the pathological degree of spinal cord injury, however no breakthrough was found within recent 20 years.
OBJECTIVE: To observe the protection effects of sodium aescinate on the nerve cell apoptosis and expression of glial fibriallary acidic protein (GFAP) in the early spinal cord injured rats.
METHODS: Spinal cord injury models were established with the modified Allen’s method in 180 Sprague-Dawley rats, and were randomly divided into three groups, with 60 rats in each group. Immediately after injury, the rats in three groups were intraperitoneally injected with sodium aescinate (5 mg/kg), methylprednisolone (100 mg/kg) and equal saline, respectively, once per day. At 8 hours, 24 hours, 96 hours and 7 days, 14 days after injury, rats were sacrificed and the injured segments were resected for hematoxylin-eosin staining and immunohistochemical staining, the nerve cell apoptosis and GFAP expression were detected.
RESULTS AND CONCLUSION: The apoptotic nerve cells were seen at 8 hours after injury and the number of apoptotic cells reached the peak at 7 days, the edema was attenuated at 14 days without less nerve cell apoptosis in all groups, significantly fewer apoptotic nerve cells can be seen in sodium aescinate and methylprednisolone groups compared with the control group (P < 0.05) at each time. The expression of GFAP was increased in the time dependant manner in all groups, the increase was slow in methylprednisolone group but sharp in sodium aescinate group and control group within 96 hours. There was no difference between control group and sodium aescinate group within 24 hours (P > 0.05), which was lower than methylprednisolone group (P < 0.05); after 96 hours, methylprednisolone group and sodium aescinate group were both significantly lower than control group (P < 0.05). Furthermore, the decreasing expression was observed in all groups after 7 days. Sodium ascinate has obvious protection effects on nerve cells in spinal cord injured rats and promotes neurological function through decreasing GFAP expression after injury. The efficacy of sodium ascinate is equal to that of methylprednisolone within 2 hours.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

全文链接：

Key words: spinal cord injuries, escin, methylprednisolone, glial fibrillary acidic protein

CLC Number:

R318

Ding Yong, Wu Yu-jie, Fu Zhi-yi, Jin Wen-jie, Hu Xiao-peng, Liu Xing-zhen. Sodium aescinate reduces glial fibriallary acidic protein expression after spinal cord injury[J]. Chinese Journal of Tissue Engineering Research, doi: 10.3969/j.issn.2095-4344.2014.15.020 .

Figures/Tables 4

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